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prism 7700 sequence detection system  (Thermo Fisher)


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    Thermo Fisher prism 7700 sequence detection system
    Prism 7700 Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+7700+sequence+detection+system/prism+7700+sequence+detection+system/pmc12096173-58-15-20
    Average 90 stars, based on 1 article reviews
    prism 7700 sequence detection system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The Utility of Long-Read Sequencing in Diagnosing Early Onset Parkinson's Disease.
    Article Snippet: .. Initially, copy number variants (CNVs) of PRKN were analyzed by quantitative polymerase chain reaction (qPCR) with TaqMan probe (Applied Biosystems, Foster City, CA) using ABI PRISM 7700 sequence detection system (Applied Biosystems, Foster City, CA) or by multiplex MLPA using SALSA MLPA Probemix P051 Parkinson mix (MRCHolland, Amsterdam, The Netherlands), as we reported previously.11,20 Second, for cases where we found either 1 variant of PRKN or PINK1 after targeted resequencing and the initial qPCR/MLPA analysis, we conducted a second MLPA experiment. .. This involved using the MLPA P052 Parkinson probe mix (MRC-Holland, Amsterdam, The Netherlands) to standardize the method for screening CNVs.

    Article Title: The Overexpression of Collagen Receptor DDR1 is Associated With Chromosome Instability and Aneuploidy in Diffuse Large B‐Cell Lymphoma
    Article Snippet: RNA was isolated from cell lines with RNeasy Mini kit including genomic DNA removal using RNase‐Free DNase Set (Qiagen Ltd), and complementary DNA (cDNA) was synthesised with qScript cDNA SuperMix (QuantaBio, Beverly, MA, USA). .. All gene transcripts were quantified by qPCR with commercial gene expression assays using the ABI Prism 7700 sequence detection system (Applied Biosystems, Thermo Fisher Scientific, Paisley, UK). ..

    Article Title: Diphenyleneiodonium chloride inhibits MYCN-amplified neuroblastoma by targeting MYCN induced mitochondrial alterations
    Article Snippet: .. Quantification of gene expression by real-time PCR was performed on an ABI Prism 7700 Sequence Detection System, (Applied Biosystems Inc., UK). .. MT-ND1, MT-ND2, MT-ND3, MT-ND4L, MT-ND6, MT-ATP6, MT-ATP8, MT-CO1, MT-CYB, TFAM, and MYCN gene expression was examined using specific Taqman gene expression assays (Applied Biosystems Inc., UK).

    Article Title: IL-32γ Induced Autophagy Through Suppression of MET and mTOR Pathways in Liver Tumor Growth Inhibition
    Article Snippet: Total RNA was isolated from tissues and cell samples using the RiboEx Total RNA (#301-001, GeneAll Biotechnology Co., Seoul, Korea) and reverse-transcribed into cDNA using High-Capacity cDNA Reverse Transcription kit (#4368813, Applied Biosystems, Foster City, CA, USA) according to manufacturer’s protocol. .. Real-time PCR was performed using the SYBR Green PCR Master Mix (#4344463, Applied Biosystems) and analyzed using an ABI PRISM 7700 Sequence Detection system (Applied Biosystems). .. All primers utilized for RT-qPCR were designed using the ‘Primer3’ on website and purchased from Bioneer Corp. (Daejeon, Republic of Korea).

    Article Title: NTRK fusion promotes tumor migration and invasion through epithelial-mesenchymal transition and closely interacts with ECM1 and NOVA1.
    Article Snippet: Finally, stable transmissible cell lines were expanded in media supplemented with drugs and verified via q‒PCR before being stored in liquid nitrogen for subsequent experiments. qRT‒PCR The Tsingzol RNA Extraction Reagent (TIANGEN Biotech, China) was utilized to extract RNA from cells and tissue samples, and cDNA was generated with Oligo (dT) Primer (Tsingke Biotechnology Co., Ltd., China) and RevertAid Reverse Transcriptase (Thermo Fisher Scientific, China). .. For all qRT‒PCR analyses, PerfectStart Green qPCR SuperMix (TransGen Biotech, China) was utilized on the ABI PRISM 7700 sequence detection system (Applied Biosystems, China). ..

    Article Title: Siglec-5 as a novel receptor mediates endothelial cells oxLDL transcytosis to promote atherosclerosis.
    Article Snippet: Background: Excessive subendothelial retention of oxidized low-density lipoprotein (oxLDL) and subsequent oxLDL engulfment by macrophages leads to the formation of foam cells and the development of atherosclerosis.. Our previous study showed that the plasma level of sialic acid-binding immunoglobulin-like lectin 5 (Siglec-5) was a novel biomarker for the prognosis of atherosclerosis in diabetic patients.. However, the role and underlying mechanisms of Siglec-5 in atherosclerosis have not been elucidated.

    Sequencing:

    Article Title: The Utility of Long-Read Sequencing in Diagnosing Early Onset Parkinson's Disease.
    Article Snippet: .. Initially, copy number variants (CNVs) of PRKN were analyzed by quantitative polymerase chain reaction (qPCR) with TaqMan probe (Applied Biosystems, Foster City, CA) using ABI PRISM 7700 sequence detection system (Applied Biosystems, Foster City, CA) or by multiplex MLPA using SALSA MLPA Probemix P051 Parkinson mix (MRCHolland, Amsterdam, The Netherlands), as we reported previously.11,20 Second, for cases where we found either 1 variant of PRKN or PINK1 after targeted resequencing and the initial qPCR/MLPA analysis, we conducted a second MLPA experiment. .. This involved using the MLPA P052 Parkinson probe mix (MRC-Holland, Amsterdam, The Netherlands) to standardize the method for screening CNVs.

    Article Title: The Overexpression of Collagen Receptor DDR1 is Associated With Chromosome Instability and Aneuploidy in Diffuse Large B‐Cell Lymphoma
    Article Snippet: RNA was isolated from cell lines with RNeasy Mini kit including genomic DNA removal using RNase‐Free DNase Set (Qiagen Ltd), and complementary DNA (cDNA) was synthesised with qScript cDNA SuperMix (QuantaBio, Beverly, MA, USA). .. All gene transcripts were quantified by qPCR with commercial gene expression assays using the ABI Prism 7700 sequence detection system (Applied Biosystems, Thermo Fisher Scientific, Paisley, UK). ..

    Article Title: Diphenyleneiodonium chloride inhibits MYCN-amplified neuroblastoma by targeting MYCN induced mitochondrial alterations
    Article Snippet: .. Quantification of gene expression by real-time PCR was performed on an ABI Prism 7700 Sequence Detection System, (Applied Biosystems Inc., UK). .. MT-ND1, MT-ND2, MT-ND3, MT-ND4L, MT-ND6, MT-ATP6, MT-ATP8, MT-CO1, MT-CYB, TFAM, and MYCN gene expression was examined using specific Taqman gene expression assays (Applied Biosystems Inc., UK).

    Article Title: IL-32γ Induced Autophagy Through Suppression of MET and mTOR Pathways in Liver Tumor Growth Inhibition
    Article Snippet: Total RNA was isolated from tissues and cell samples using the RiboEx Total RNA (#301-001, GeneAll Biotechnology Co., Seoul, Korea) and reverse-transcribed into cDNA using High-Capacity cDNA Reverse Transcription kit (#4368813, Applied Biosystems, Foster City, CA, USA) according to manufacturer’s protocol. .. Real-time PCR was performed using the SYBR Green PCR Master Mix (#4344463, Applied Biosystems) and analyzed using an ABI PRISM 7700 Sequence Detection system (Applied Biosystems). .. All primers utilized for RT-qPCR were designed using the ‘Primer3’ on website and purchased from Bioneer Corp. (Daejeon, Republic of Korea).

    Article Title: NTRK fusion promotes tumor migration and invasion through epithelial-mesenchymal transition and closely interacts with ECM1 and NOVA1.
    Article Snippet: Finally, stable transmissible cell lines were expanded in media supplemented with drugs and verified via q‒PCR before being stored in liquid nitrogen for subsequent experiments. qRT‒PCR The Tsingzol RNA Extraction Reagent (TIANGEN Biotech, China) was utilized to extract RNA from cells and tissue samples, and cDNA was generated with Oligo (dT) Primer (Tsingke Biotechnology Co., Ltd., China) and RevertAid Reverse Transcriptase (Thermo Fisher Scientific, China). .. For all qRT‒PCR analyses, PerfectStart Green qPCR SuperMix (TransGen Biotech, China) was utilized on the ABI PRISM 7700 sequence detection system (Applied Biosystems, China). ..

    Article Title: Sesamin induces cell cycle arrest and upregulation of NKG2D ligands in MG‑63 cells and increases susceptibility to NK cell cytotoxicity
    Article Snippet: Reverse transcription was performed using SuperScript IV VILO Master Mix (cat. no. 11756050; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. Quantification of mRNA levels of the genes of interest was performed using the ABI PRISM 7700 sequence detection system (Applied Biosystems; Thermo Fisher Scientific, Inc.) with designated primer pairs. .. FastStart Universal SYBR Green Master (Roche Applied Science) was used for qPCR.

    Article Title: Combination Treatment of Resistant Acute Promyelocytic Leukemia Cells with Arsenic Trioxide and Anti-Apoptotic Gene Inhibitors
    Article Snippet: .. The qRT-PCR reactions and fluorescence measurements were conducted on the ABI PRISM 7700 Sequence Detection System (Thermo Fisher Scientific). ..

    Article Title: Siglec-5 as a novel receptor mediates endothelial cells oxLDL transcytosis to promote atherosclerosis.
    Article Snippet: Background: Excessive subendothelial retention of oxidized low-density lipoprotein (oxLDL) and subsequent oxLDL engulfment by macrophages leads to the formation of foam cells and the development of atherosclerosis.. Our previous study showed that the plasma level of sialic acid-binding immunoglobulin-like lectin 5 (Siglec-5) was a novel biomarker for the prognosis of atherosclerosis in diabetic patients.. However, the role and underlying mechanisms of Siglec-5 in atherosclerosis have not been elucidated.

    Multiplex Assay:

    Article Title: The Utility of Long-Read Sequencing in Diagnosing Early Onset Parkinson's Disease.
    Article Snippet: .. Initially, copy number variants (CNVs) of PRKN were analyzed by quantitative polymerase chain reaction (qPCR) with TaqMan probe (Applied Biosystems, Foster City, CA) using ABI PRISM 7700 sequence detection system (Applied Biosystems, Foster City, CA) or by multiplex MLPA using SALSA MLPA Probemix P051 Parkinson mix (MRCHolland, Amsterdam, The Netherlands), as we reported previously.11,20 Second, for cases where we found either 1 variant of PRKN or PINK1 after targeted resequencing and the initial qPCR/MLPA analysis, we conducted a second MLPA experiment. .. This involved using the MLPA P052 Parkinson probe mix (MRC-Holland, Amsterdam, The Netherlands) to standardize the method for screening CNVs.

    Multiplex Ligation-dependent Probe Amplification:

    Article Title: The Utility of Long-Read Sequencing in Diagnosing Early Onset Parkinson's Disease.
    Article Snippet: .. Initially, copy number variants (CNVs) of PRKN were analyzed by quantitative polymerase chain reaction (qPCR) with TaqMan probe (Applied Biosystems, Foster City, CA) using ABI PRISM 7700 sequence detection system (Applied Biosystems, Foster City, CA) or by multiplex MLPA using SALSA MLPA Probemix P051 Parkinson mix (MRCHolland, Amsterdam, The Netherlands), as we reported previously.11,20 Second, for cases where we found either 1 variant of PRKN or PINK1 after targeted resequencing and the initial qPCR/MLPA analysis, we conducted a second MLPA experiment. .. This involved using the MLPA P052 Parkinson probe mix (MRC-Holland, Amsterdam, The Netherlands) to standardize the method for screening CNVs.

    Variant Assay:

    Article Title: The Utility of Long-Read Sequencing in Diagnosing Early Onset Parkinson's Disease.
    Article Snippet: .. Initially, copy number variants (CNVs) of PRKN were analyzed by quantitative polymerase chain reaction (qPCR) with TaqMan probe (Applied Biosystems, Foster City, CA) using ABI PRISM 7700 sequence detection system (Applied Biosystems, Foster City, CA) or by multiplex MLPA using SALSA MLPA Probemix P051 Parkinson mix (MRCHolland, Amsterdam, The Netherlands), as we reported previously.11,20 Second, for cases where we found either 1 variant of PRKN or PINK1 after targeted resequencing and the initial qPCR/MLPA analysis, we conducted a second MLPA experiment. .. This involved using the MLPA P052 Parkinson probe mix (MRC-Holland, Amsterdam, The Netherlands) to standardize the method for screening CNVs.

    Gene Expression:

    Article Title: The Overexpression of Collagen Receptor DDR1 is Associated With Chromosome Instability and Aneuploidy in Diffuse Large B‐Cell Lymphoma
    Article Snippet: RNA was isolated from cell lines with RNeasy Mini kit including genomic DNA removal using RNase‐Free DNase Set (Qiagen Ltd), and complementary DNA (cDNA) was synthesised with qScript cDNA SuperMix (QuantaBio, Beverly, MA, USA). .. All gene transcripts were quantified by qPCR with commercial gene expression assays using the ABI Prism 7700 sequence detection system (Applied Biosystems, Thermo Fisher Scientific, Paisley, UK). ..

    Article Title: Diphenyleneiodonium chloride inhibits MYCN-amplified neuroblastoma by targeting MYCN induced mitochondrial alterations
    Article Snippet: .. Quantification of gene expression by real-time PCR was performed on an ABI Prism 7700 Sequence Detection System, (Applied Biosystems Inc., UK). .. MT-ND1, MT-ND2, MT-ND3, MT-ND4L, MT-ND6, MT-ATP6, MT-ATP8, MT-CO1, MT-CYB, TFAM, and MYCN gene expression was examined using specific Taqman gene expression assays (Applied Biosystems Inc., UK).

    SYBR Green Assay:

    Article Title: IL-32γ Induced Autophagy Through Suppression of MET and mTOR Pathways in Liver Tumor Growth Inhibition
    Article Snippet: Total RNA was isolated from tissues and cell samples using the RiboEx Total RNA (#301-001, GeneAll Biotechnology Co., Seoul, Korea) and reverse-transcribed into cDNA using High-Capacity cDNA Reverse Transcription kit (#4368813, Applied Biosystems, Foster City, CA, USA) according to manufacturer’s protocol. .. Real-time PCR was performed using the SYBR Green PCR Master Mix (#4344463, Applied Biosystems) and analyzed using an ABI PRISM 7700 Sequence Detection system (Applied Biosystems). .. All primers utilized for RT-qPCR were designed using the ‘Primer3’ on website and purchased from Bioneer Corp. (Daejeon, Republic of Korea).

    Article Title: Siglec-5 as a novel receptor mediates endothelial cells oxLDL transcytosis to promote atherosclerosis.
    Article Snippet: Background: Excessive subendothelial retention of oxidized low-density lipoprotein (oxLDL) and subsequent oxLDL engulfment by macrophages leads to the formation of foam cells and the development of atherosclerosis.. Our previous study showed that the plasma level of sialic acid-binding immunoglobulin-like lectin 5 (Siglec-5) was a novel biomarker for the prognosis of atherosclerosis in diabetic patients.. However, the role and underlying mechanisms of Siglec-5 in atherosclerosis have not been elucidated.

    Polymerase Chain Reaction:

    Article Title: IL-32γ Induced Autophagy Through Suppression of MET and mTOR Pathways in Liver Tumor Growth Inhibition
    Article Snippet: Total RNA was isolated from tissues and cell samples using the RiboEx Total RNA (#301-001, GeneAll Biotechnology Co., Seoul, Korea) and reverse-transcribed into cDNA using High-Capacity cDNA Reverse Transcription kit (#4368813, Applied Biosystems, Foster City, CA, USA) according to manufacturer’s protocol. .. Real-time PCR was performed using the SYBR Green PCR Master Mix (#4344463, Applied Biosystems) and analyzed using an ABI PRISM 7700 Sequence Detection system (Applied Biosystems). .. All primers utilized for RT-qPCR were designed using the ‘Primer3’ on website and purchased from Bioneer Corp. (Daejeon, Republic of Korea).

    Quantitative RT-PCR:

    Article Title: Combination Treatment of Resistant Acute Promyelocytic Leukemia Cells with Arsenic Trioxide and Anti-Apoptotic Gene Inhibitors
    Article Snippet: .. The qRT-PCR reactions and fluorescence measurements were conducted on the ABI PRISM 7700 Sequence Detection System (Thermo Fisher Scientific). ..

    Fluorescence:

    Article Title: Combination Treatment of Resistant Acute Promyelocytic Leukemia Cells with Arsenic Trioxide and Anti-Apoptotic Gene Inhibitors
    Article Snippet: .. The qRT-PCR reactions and fluorescence measurements were conducted on the ABI PRISM 7700 Sequence Detection System (Thermo Fisher Scientific). ..



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